Genotyping and primer details


Genotyping and primer details for SALK_021211c - At1g29710
Line ID SALK_021211c
Locus At1g29710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10388627 of line SALK_021211c Chr1:10388627
   
Insertion allele amplicon LBb3 - 07rr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 07rr: 5'-TCGTTTTAAAGAAGAAGGGAACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10388299
   
wt amplicon (over InsPos) 12sx - 07rr
wt amplicon start Chr1:10388299
wt amplicon end Chr1:10389057
wt amplicon size 759 bp
Locus specific primer 07rr: 5'-TCGTTTTAAAGAAGAAGGGAACAA-3'
2nd locus specific primer 12sx: 5'-TGGTATATCTTTGCATCTCGCTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11