Sequences from confirmation amplicon


Confirmation sequences for line SALK_021211c and hit At1g29710 are shown.
Line ID SALK_021211c
Locus At1g29710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10388627
Primers used for PCR 07rr - LBb3, show primer details
   
Sequence Name 41-K116392-SALK_021211C-07rr
GenBank Accession LN485316 [GenBank]
Sequencing primer gene specific primer, 07rr: 5'-TCGTTTTAAAGAAGAAGGGAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K116392-SALK_021211C-07rr
CAGGAGATGTTAAGGAAGGGTCTCTGCAATTTTCAAGCTATGTACAGAGAATATGGAATC
GTACCTTCAATGGAACACTATCATAGTGTTACCAAAATGTTGGCAACATCTGGTCACTTG
GATGAGGCACTGAACTTTGTTGAGAGAATGCCGATGGAACCGAGTGTTGACGTGTGGGAA
ACACTAATGAATCTTTCTCGGGTTCATGGAGATGTAGAGCTTGGAGATAGATGTGCTGAG
TTGGTTGAGAAGTTAGATGCCACAAGATTAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGA
AAATCCTGTTTGATGGTGGTTCCA
   
Sequence Name 16-K116391-SALK_021211C-R210
GenBank Accession LN484725 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116391-SALK_021211C-R210
CATCTACTTCTCACCAACTCAGCACATCTATCTCCAAGCTCTACATCTCCATGAACCCGA
GAAAGATTCATTAGTGTTTCCCACACGTCAACACTCGGTTCCATCGGCATTCTCTCAACA
AAGTTCAGTGCCTCATCCAAGTGACCAGATGTTGCCAACATTTTGGTAACACTATGATAG
TGTTCCATTGAAGGTACGATTCCATATTCTCTGTACATAGCTTGAAATTGCAGAGACCCT
TCCTTAACATCTCCTGTCAAAGTACATGTAGAAAACACTTGGTTGAATATCTCTCCGTTT
GGTTTGTTCCCTTCTTCTTTAAAACGAA

Last Updated on 06.12.2018 16:11