Genotyping and primer details


Genotyping and primer details for SALK_021513 - At2g20000
Line ID SALK_021513
Locus At2g20000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8636499 of line SALK_021513 Chr2:8636499
   
Insertion allele amplicon LBb3 - 41fp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 41fp: 5'-GAAGAAAGGCAACAACTATGGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8636917
   
wt amplicon (over InsPos) 09go - 41fp
wt amplicon start Chr2:8635908
wt amplicon end Chr2:8636917
wt amplicon size 1010 bp
Locus specific primer 41fp: 5'-GAAGAAAGGCAACAACTATGGAAG-3'
2nd locus specific primer 09go: 5'-GAAGCATGATAATGCGAACAAGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11