Sequences from confirmation amplicon


Confirmation sequences for line SALK_021513 and hit At2g20000 are shown.
Line ID SALK_021513
Locus At2g20000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8636499
Primers used for PCR 41fp - LBb3, show primer details
   
Sequence Name 77-K111509-SALK_021513C-41fp
GenBank Accession LN486171 [GenBank]
Sequencing primer gene specific primer, 41fp: 5'-GAAGAAAGGCAACAACTATGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K111509-SALK_021513C-41fp
CTTCGTATTTTGTCTACAAAAATGCTATTTTCATGTGCGAGCGTCTCTGCGCTGAGTTTC
CTTCTGAGGTAATCACCCTCTTCTTTCACTCTCTCTCTCTGATTTTACCTCTCTAATTCA
AATTCTGTAAATCGAAGCTCTTGGAATGGTAAATTTGATATTTTTGGGTTTGTAATTCCT
CTGGGTATCTATGAATTCGTCGAAAGTGCGTCTCTTTTTGGATTTGGAATTCGATAGCTT
CACTGTGTTCTTCGAGATTGATTTTGGTTTCTTACCTTTTAGCCCTTTGTTTTCAAGATC
CGTGTGTTCAATTAGGAGATGAATTCGTGTTCTTTTCTCTCTCTTGTTGAATTTGTTTTC
TCTAGAAAATTTGCATTTTGAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
ACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 63-K111508-SALK_021513C-R210
GenBank Accession LN485820 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K111508-SALK_021513C-R210
TCATTTGTTTACCCACAATATATCCTCAAATGCAAATTTTCTAGAGAAAACAAATTCAAC
AAGAGAGAGAAAAGAACACGAATTCATCTCCTAATTGAACACACGGATCTTGAAAACAAA
GGGCTAAAAGGTAAGAAACCAAAATCAATCTCGAAGAACACAGTGAAGCTATCGAATTCC
AAATCCAAAAAGAGACGCACTTTCGACGAATTCATAGATACCCAGAGGAATTACAAACCC
AAAAATATCAAATTTACCATTCCAAGAGCTTCGATTTACAGAATTTGAATTAGAGAGGTA
AAATCAGAGAGAGAGAGTGAAAGAAGAGGGTGATTACCTCAGAAGGAAACTCAGCGCAGA
GACGCTCGCACATGAAAATAGCATTTTTGTAGACAAAATGACGAAGACTGTTGTTTACAC
AGTCCACAAGCATAGCTTCCATAGTTGTTGCCTTTCTT

Last Updated on 06.12.2018 16:11