Genotyping and primer details


Genotyping and primer details for SALK_021788c - At2g41160
Line ID SALK_021788c
Locus At2g41160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17157133 of line SALK_021788c Chr2:17157133
   
Insertion allele amplicon LBb3 - 18ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18ht: 5'-CTTCCGAGTCTTTGAGAGACAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17157808
   
wt amplicon (over InsPos) 07ix - 18ht
wt amplicon start Chr2:17156785
wt amplicon end Chr2:17157808
wt amplicon size 1024 bp
Locus specific primer 18ht: 5'-CTTCCGAGTCTTTGAGAGACAGAT-3'
2nd locus specific primer 07ix: 5'-GTTTGTTTTTAAGCAAGAGTTGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11