Sequences from confirmation amplicon


Confirmation sequences for line SALK_021788c and hit At2g41160 are shown.
Line ID SALK_021788c
Locus At2g41160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17157133
Primers used for PCR 18ht - LBb3, show primer details
   
Sequence Name 76-K112669-SALK_021788C-18ht
GenBank Accession LN486151 [GenBank]
Sequencing primer gene specific primer, 18ht: 5'-CTTCCGAGTCTTTGAGAGACAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K112669-SALK_021788C-18ht
CTCAGTTTTTTCTTCTGACCTTTTTTTGTCTATTATTTTGGTAAATAGCATGATACCTGC
TGATATTTCCTTTGAGATTGAAATTCTATGAGTTAATATGTGCTCATTCTGCAGGTTTTC
ATCTTCTTCTCTGGATTTGTATCACTAATACTAGAGACCATTTTGTTATCATTGACTAAA
GGTAAACCTTCCTATTTTCAGATAGTCTCTTCAAAAACTAGACCGGTTTGATATGATTTA
CCTTTATGATATGATTTGGGGGCGCTAATGCAGACAACTATATGGGATTGTAGTTATTAG
TTAGCCTTGTTACTAGTCTTTCCCCACTCATCGAAGGAAAACTGTGAAGGGAGGATTGGG
ATATTGTGACGATCTTTGTTGGTTTCAAATTCTGACCTGCAATTTCCTTCACTCATACTA
TTGAAAATATCTTATAAAGAATTCCCTTGGTCCATCATTTAAATTATCAAGAAATGCTTT
TTGTGCATTCTTAATAAGCATTTTTGATATGACCTTTTATACACTCCGTATCACAAACAC
TTTACAGTTATTGATAGTGGGTATATAACAAACTGCTGTCTTTTTTGTAGATCCTACTGC
AAACCTACTGACATCTTAATAACAAATTGACGCTNAGACAACTTAATAACACATTGCGGA
CGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTG
CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTNTGCCCCAGC
AGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 66-K112668-SALK_021788C-R210
GenBank Accession LN485900 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K112668-SALK_021788C-R210
TTTGTTATAAGATGTCAGTAGGTTTGCAGTAGGATCTACAAAAAAGACAGCAGTTTGTTA
TATACCCACTATCAATAACTGTAAAGTGTTTGTGATACGGAGTGTATAAAAGGTCATATC
AAAAATGCTTATTAAGAATGCACAAAAAGCATTTCTTGATAATTTAAATGATGGACCAAG
GGAATTCTTTATAAGATATTTTCAATAGTATGAGTGAAGGAAATTGCAGGTCAGAATTTG
AAACCAACAAAGATCGTCACAATATCCCAATCCTCCCTTCACAGTTTTCCTTCGATGAGT
GGGGAAAGACTAGTAACAAGGCTAACTAATAACTACAATCCCATATAGTTGTCTGCATTA
GCGCCCCCAAATCATATCATAAAGGTAAATCATATCAAACCGGTCTAGTTTTTGAAGAGA
CTATCTGAAAATAGGAAGGTTTACCTTTAGTCAATGATAACAAAATGGTCTCTAGTATTA
GTGATACAAATCCAGAGAAGAAGATGAAAACCTGCAGAATGAGCACATATTAACTCATAG
AATTTCAATCTCAAAGGAAATATCAGCAGGTATCTTGCTATTTACCAAAATAATAGACAA
AAAAAGGTCATGAAGAAAAAACTGAGCTAGACTATCCCTTACCGAATACTTATTTGAACC
AATCTGTCTCTCANAGACTCGGA

Last Updated on 06.12.2018 16:11