Genotyping and primer details


Genotyping and primer details for SALK_021811c - At1g10410
Line ID SALK_021811c
Locus At1g10410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3417715 of line SALK_021811c Chr1:3417715
   
Insertion allele amplicon LBb3 - 40cp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 40cp: 5'-GAGAAGGTCTCTTAGCTCTAGCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3418313
   
wt amplicon (over InsPos) 93dy - 40cp
wt amplicon start Chr1:3417393
wt amplicon end Chr1:3418313
wt amplicon size 921 bp
Locus specific primer 40cp: 5'-GAGAAGGTCTCTTAGCTCTAGCCC-3'
2nd locus specific primer 93dy: 5'-GGAAGCCTTTAACTTTCTCAACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11