Genotyping and primer details


Genotyping and primer details for SALK_021893c - At5g25360
Line ID SALK_021893c
Locus At5g25360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:8800106 of line SALK_021893c Chr5:8800106
   
Insertion allele amplicon LBb3 - 35wu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 35wu: 5'-CGCTCTGTTTATTCGTATTTTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:8800935
   
wt amplicon (over InsPos) 07xf - 35wu
wt amplicon start Chr5:8799531
wt amplicon end Chr5:8800935
wt amplicon size 1405 bp
Locus specific primer 35wu: 5'-CGCTCTGTTTATTCGTATTTTGGT-3'
2nd locus specific primer 07xf: 5'-TTCTGAGTGTAACAAAAATCGACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11