Genotyping and primer details


Genotyping and primer details for SALK_022192c - At5g18240
Line ID SALK_022192c
Locus At5g18240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6028728 of line SALK_022192c Chr5:6028728
   
Insertion allele amplicon LBb3 - 36dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 36dh: 5'-AATGTTTGGTTAAGTGGTCCAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6028043
   
wt amplicon (over InsPos) 94dy - 36dh
wt amplicon start Chr5:6028043
wt amplicon end Chr5:6029135
wt amplicon size 1093 bp
Locus specific primer 36dh: 5'-AATGTTTGGTTAAGTGGTCCAAAG-3'
2nd locus specific primer 94dy: 5'-GAACTGCAGAATCTACACCATCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11