Genotyping and primer details


Genotyping and primer details for SALK_022805c - At5g40860
Line ID SALK_022805c
Locus At5g40860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16370678 of line SALK_022805c Chr5:16370678
   
Insertion allele amplicon LBb3 - 10rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 10rb: 5'-CAGCCATTGATTAGTGGAAGAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16371151
   
wt amplicon (over InsPos) 23rz - 10rb
wt amplicon start Chr5:16370222
wt amplicon end Chr5:16371151
wt amplicon size 930 bp
Locus specific primer 10rb: 5'-CAGCCATTGATTAGTGGAAGAAAT-3'
2nd locus specific primer 23rz: 5'-ACGACATCGTGTATTTTATCTCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11