Sequences from confirmation amplicon


Confirmation sequences for line SALK_022805c and hit At5g40860 are shown.
Line ID SALK_022805c
Locus At5g40860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16370678
Primers used for PCR 10rb - LBb3, show primer details
   
Sequence Name 67-K116055-SALK_022805C-10rb
GenBank Accession LN485937 [GenBank]
Sequencing primer gene specific primer, 10rb: 5'-CAGCCATTGATTAGTGGAAGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K116055-SALK_022805C-10rb
TTTGAGAGAAGAAAGCCAATGATTAGATAAATCCAGTTAAGACGATGATATGATTATGAT
TTTAATATAAAGAAAATTCGTTCTTCGAATCTATTAATGGAAACAAAATCAATGAAATTA
ACGCTAAGAATTCAGGATATTATCTTACTACAAAGTACAAAGAGCTCAATTATGATATTT
TCACACCATCAAAGAAAACAAAAATATTCTTTATAATCAGATTACCATAAAAGGAAATTG
AAAACAACAAAAAAAAAGATTATGAAACAAGTACCAAAGCTTCTCTTAGAAGAAGAAAAC
GACCACCGAGATTTCACTTGAGCACTTAAACCATTATCCACCGTCTTCGGAGTAGCCACC
GGAGCTAAATTCTCCTTTACCGGCGATTCTGAAGGTAAAGTATGAATAAACTCATATATA
TACGTACGTTTTTCTTACGCTTAAACTATCAGGATATATTGTGGTGTAAACAAATTGACG
CTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTT
CACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
CAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 40-K116054-SALK_022805C-R210
GenBank Accession LN485296 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K116054-SALK_022805C-R210
ATATATGAGTTTATTCATACTTTACCTTCAGAATCGCCGGTAAAGGAGAATTTAGCTCCG
GTGGCTACTCCAAAGACGGTGGATAATGGTTTAATTGCTCAAGTGAAATCTCGGTGGTCG
TTTTCTTCTTCTAAGAGAAGCTTTGGTACTTGTTTCATAATCTTTTTTTTTGTTGTTTTC
AATTTCCTTTGATGGTAATCTGATTATAAAGAATATTTTTGTTTTCTTTGATGGTGTGAA
AATATCAAAATTGAGCTCTTTGTACTTTGTAATAAAATAATATCCTGAATTCTTAGCGTT
AATTTCATTGATTTTGTTTCCTTTAATAGATTCGAAGAAGGAATTTTCTTAAAAAAAAAA
TCATAATCATATCATCGTCTTAACTGGATTTATCTAATCATTGGCTTTTCTTCTCGACAA
AATAGAGAGATTTATCTTTTTCCAAAATAGATTTCTTCCACTAATCAGTGGGG

Last Updated on 06.12.2018 16:11