Genotyping and primer details


Genotyping and primer details for SALK_023159c - At1g80300
Line ID SALK_023159c
Locus At1g80300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30192936 of line SALK_023159c Chr1:30192936
   
Insertion allele amplicon LBb3 - 50us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 50us: 5'-ATACCAAGAACTTCAAGCTTTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30193335
   
wt amplicon (over InsPos) 34vl - 50us
wt amplicon start Chr1:30192413
wt amplicon end Chr1:30193335
wt amplicon size 923 bp
Locus specific primer 50us: 5'-ATACCAAGAACTTCAAGCTTTCCA-3'
2nd locus specific primer 34vl: 5'-TTCTTGAAGACTTGGGTGAATCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11