Genotyping and primer details


Genotyping and primer details for SALK_023188 - At3g06140
Line ID SALK_023188
Locus At3g06140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1858214 of line SALK_023188 Chr3:1858214
   
Insertion allele amplicon LBb3 - 10vt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 10vt: 5'-GCGGATACACATCTTCTTCTAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1857675
   
wt amplicon (over InsPos) 72wi - 10vt
wt amplicon start Chr3:1857675
wt amplicon end Chr3:1858836
wt amplicon size 1162 bp
Locus specific primer 10vt: 5'-GCGGATACACATCTTCTTCTAAGG-3'
2nd locus specific primer 72wi: 5'-CTTCTCACTGTTGTGCTTTCTGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11