Genotyping and primer details


Genotyping and primer details for SALK_023251c - At3g21160
Line ID SALK_023251c
Locus At3g21160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7417271 of line SALK_023251c Chr3:7417271
   
Insertion allele amplicon R204 - 43ai
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 43ai: 5'-GGCGACTATCTCGATAAAGTTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7416414
   
wt amplicon (over InsPos) 02lt - 43ai
wt amplicon start Chr3:7416414
wt amplicon end Chr3:7417563
wt amplicon size 1150 bp
Locus specific primer 43ai: 5'-GGCGACTATCTCGATAAAGTTTTG-3'
2nd locus specific primer 02lt: 5'-CTGGGGACAAGATGAGCTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11