Genotyping and primer details


Genotyping and primer details for SALK_023492 - At5g05700
Line ID SALK_023492
Locus At5g05700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1714163 of line SALK_023492 Chr5:1714163
   
Insertion allele amplicon R204 - CF58
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CF58: 5'-CTTTGAAACATGCATATGAGGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1714963
   
wt amplicon (over InsPos) CF59 - CF58
wt amplicon start Chr5:1713762
wt amplicon end Chr5:1714963
wt amplicon size 1202 bp
Locus specific primer CF58: 5'-CTTTGAAACATGCATATGAGGAGA-3'
2nd locus specific primer CF59: 5'-TGGACGATAGGCTGCTTTATATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11