Sequences from confirmation amplicon


Confirmation sequences for line SALK_023492 and hit At5g05700 are shown.
Line ID SALK_023492
Locus At5g05700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1714163
Primers used for PCR CF58 - R204, show primer details
   
Sequence Name 50-K107203-SALK_023492C-CF58
GenBank Accession LN485497 [GenBank]
Sequencing primer gene specific primer, CF58: 5'-CTTTGAAACATGCATATGAGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K107203-SALK_023492C-CF58
GATCTTCAGATTTCTCTAATGAAGTCGTTCATTGAAGAGCTTCCTGAATTTTCTTAATAA
TGTTTTGTCAAAGGTTCTTGGATGGCAAACTAGATGTGCAGCCCAGGGAACAAAGAGGTG
CTTCTTCCTCTGGTGATGTTTCAGATACAAGGAGAAAAACACTTGGAGCTGCTAAAAGTG
AAGAGAATAAGAAAGTTGAAGCAGTTATGGATGATTTGTCTAAAAATATTGACCAAGCTG
TGCAATTATGCATACGGAGTGGGGAATTCCCGTCTAATATGCAGATACCAAAAGCTTCAG
TGAAGAAGGTTTTCTGTGCTAGAAGAAAGAAACTAGCTGAAGGAACAGAACAAATCTTAT
ACACCAGCAACATTGCTTTTCCAATAGCAGCTGCAATAAAACGCATCCAGACATCTGAGA
AAGAGGGGATAAATAGTGCAGAAGGAAATAGATTATCACCTGAAACTATTTCTGAGATGT
TGTTGAGTGCAATGCATAAGGTGGGGGAAACTCCTGATGTGTCTATCAAAGTCTGTAAGG
GCCATATCAATTTCCTTTCATCTGCCAAAGATTCTTTTTCTGATAGAGATGTTGTACCTA
ATGGTAACATCTCGAGAGGAGCCAATTCTTTAGATGGAAGTGAAACCCTTCACGCTAAGA
AGGATTCAGAAAACCATCAGGCAAGAAAGCGAAAGCTGGAGATACATTTGAAAAGGTCTA
GTTTTGATCCTGAAGAACATGAGTTATACAAACAAATTGACGCTNAGACAACTTAATAAC
ACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAA
CAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
   
Sequence Name 42-K107202-SALK_023492C-R210
GenBank Accession LN485326 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K107202-SALK_023492C-R210
GCGTCATTTGTTTGATACTCATGTTCTTCAGGATCAAAACTAGACCTTTTCAAATGTATC
TCCAGCTTTCGCTTTCTTGCCTGATGGTTTTCTGAATCCTTCTTAGCGTGAAGGGTTTCA
CTTCCATCTAAAGAATTGGCTCCTCTCGAGATGTTACCATTAGGTACAACATCTCTATCA
GAAAAAGAATCTTTGGCAGATGAAAGGAAATTGATATGGCCCTTACAGACTTTGATAGAC
ACATCAGGAGTTTCCCCCACCTTATGCATTGCACTCAACAACATCTCAGAAATAGTTTCA
GGTGATAATCTATTTCCTTCTGCACTATTTATCCCCTCTTTCTCAGATGTCTGGATGCGT
TTTATTGCAGCTGCTATTGGAAAAGCAATGTTGCTGGTGTATAAGATTTGTTCTGTTCCT
TCAGCTAGTTTCTTTCTTCTAGCACAGAAAACCTTCTTCACTGAAGCTTTTGGTATCTGC
ATATTAGACGGGAATTCCCCACTCCGTATGCATAATTGCACAGCTTGGTCAATATTTTTA
GACAAATCATCCATAACTGCTTCAACTTTCTTATTCTCTTCACTTTTAGCAGCTCCAAGT
GTTTTTCTCCTTGTATCTGAAACATCACCAGAGGAAGAAGCACCTCTTTGTTCCCTGGGC
TGCACATCTAGTTTGCCATCCAAGAACCTTTGACAAAACATTATTAAGAAAATTCAGGAA
GCTCTTCAATGAACGACTTCATTAGAGAAATCTGAAGATCGTGAAGAATATTTACACATC
AGAATCATAGATCTCTCCTCATAT

Last Updated on 06.12.2018 16:11