Genotyping and primer details


Genotyping and primer details for SALK_023637 - At2g32290
Line ID SALK_023637
Locus At2g32290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13715994 of line SALK_023637 Chr2:13715994
   
Insertion allele amplicon LBb3 - 74by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 74by: 5'-TGACCAACTATGTTCCTGTCTACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13716601
   
wt amplicon (over InsPos) 05dd - 74by
wt amplicon start Chr2:13715371
wt amplicon end Chr2:13716601
wt amplicon size 1231 bp
Locus specific primer 74by: 5'-TGACCAACTATGTTCCTGTCTACG-3'
2nd locus specific primer 05dd: 5'-CACGATAACCATCTCTGTTTTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11