Genotyping and primer details


Genotyping and primer details for SALK_023915c - At4g13940
Line ID SALK_023915c
Locus At4g13940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8056278 of line SALK_023915c Chr4:8056278
   
Insertion allele amplicon LBb3 - 68xo
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 68xo: 5'-ACTCTTTTGATTCATGAGGGTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8055360
   
Insertion allele amplicon R212 - CE24
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer CE24: 5'-GTAATGCAACCTTCTCATCCAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8056566
   
wt amplicon (over InsPos) CE23 - CE24
wt amplicon start Chr4:8055556
wt amplicon end Chr4:8056566
wt amplicon size 1011 bp
Locus specific primer CE24: 5'-GTAATGCAACCTTCTCATCCAAAT-3'
2nd locus specific primer CE23: 5'-GTGTTAAGAGGCTTTACCAGATGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11