Genotyping and primer details


Genotyping and primer details for SALK_024059c - At3g06490
Line ID SALK_024059c
Locus At3g06490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2004895 of line SALK_024059c Chr3:2004895
   
Insertion allele amplicon LBb3 - 44lu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 44lu: 5'-CTTGAAGAACAACAACATGGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2004318
   
wt amplicon (over InsPos) 14mu - 44lu
wt amplicon start Chr3:2004318
wt amplicon end Chr3:2005194
wt amplicon size 877 bp
Locus specific primer 44lu: 5'-CTTGAAGAACAACAACATGGAAGA-3'
2nd locus specific primer 14mu: 5'-GGCCTGTTTCATTGGTAAAAGATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11