Sequences from confirmation amplicon


Confirmation sequences for line SALK_024059c and hit At3g06490 are shown.
Line ID SALK_024059c
Locus At3g06490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2004895
Primers used for PCR 44lu - LBb3, show primer details
   
Sequence Name 57-K114145-SALK_024059C-R210
GenBank Accession LN485668 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K114145-SALK_024059C-R210
CAAAATTTTATTCAATTTGAGATGGTTAATAATTGAATCCATTACCAAAAGATCTCACTC
AAATCATCAATACTTTATTTATTGGAAGGAAATTATATTTAGAAATAAGAAGAAGACGAG
AAAGAGAAAACATGAGAGGTATGCACTTTTTGATTCATACCTATGAAGGGTCTTTTCATA
TATGCATATAGGGAAAGAAACCATTTTCGAATAATAATTTTTTCTAAAAACTATCTTTAA
AAATAGTAAAGGTTGGGCAATTTAAAAGAAAAAAAAAAACCCAAATCACCCATGGTGATT
GAAACATGAAAAGGATTGAACAAAAAGCAATAAATCAATTATGTTATGCGACTTGTTCTT
TGGCTCTTTATGCTTAAAAAATGCTCAGGGAAGTGGAAAATAGATTTTTACAACTATTTT
ATCTGTATATGAAAATATATTTCAAACCGGCGCAACGAGAAAGAGAGTTCCAGCGACCTT
CTCCTTTAGTAGCAATGTAATTCAGGAGCTTAAAATCTTCTTCAGCAGTCCACGGACCTC
TTTTTAGGTCCATCTCGTCTTCCAGGT
   
Sequence Name 68-K114146-SALK_024059C-44lu
GenBank Accession LN485959 [GenBank]
Sequencing primer gene specific primer, 44lu: 5'-CTTGAAGAACAACAACATGGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K114146-SALK_024059C-44lu
TGAGAGATTTAAGCTCATGAATTACATTGCTACTAATGGAGAAGGTCGCTGGAACTCTCT
TTCTCGTTGCGCCGGTATGATATATATTTTCATATACAGATAATATAGTTGTATATATCT
ATTTTCCACTTCCATGAGCATCTTTTAAGCATAAAGAGCCAAAGAACAAGTCGCATAACA
TAATTGATTTATTGCTTTTTGTTCAATCCTTTTCATGTTTCAATCACCATGTGTGATTTG
TGTTTTTTTTTTTCTTTAAAATTGCCCAACCTTTACAATTTTTAAAGATAGTTTTTAAAA
AAAATTATAATTCGAAAAGGGTTTCTTTCCCTATATGCATATTTAAAAAGACCCTTCAAA
GGTTTGAATCAAAAAGTGCATACCTCTAAGGTTTTCCCTTTCCCGCCTTCTTCTAATTTC
TAAATAAAATTTCCTTCCAATAAATAAGGTATTGATGATTTGAGTGAAATCTTTGGGTAA
GGGATTCAATTATAAACCATCTCAAATTTGAATAAAATTTTGGTGTAAACAAATTGACGC
TAAAACAACTTAATAACACATTGCGGACGTTTTTAAGGTACGGGGGTGGTTTTTCTTTTC
ACCAGTGAAACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCCCTAAAAAATTTGCACC
AAGCGGTCCACGCTGGTTTGCCCCACCAGGCGAAAATCCGGTTTGATGGTGGTTCCA

Last Updated on 06.12.2018 16:11