Genotyping and primer details


Genotyping and primer details for SALK_024241c - At1g33060
Line ID SALK_024241c
Locus At1g33060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11978007 of line SALK_024241c Chr1:11978007
   
Insertion allele amplicon R204 - CF50
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CF50: 5'-TCAAAGGTCTGTCTCTTCAGGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11978522
   
wt amplicon (over InsPos) CF51 - CF50
wt amplicon start Chr1:11977675
wt amplicon end Chr1:11978522
wt amplicon size 848 bp
Locus specific primer CF50: 5'-TCAAAGGTCTGTCTCTTCAGGTTT-3'
2nd locus specific primer CF51: 5'-AAGCCAAAAGGTCACTATCAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11