Genotyping and primer details


Genotyping and primer details for SALK_024253 - At5g16540
Line ID SALK_024253
Locus At5g16540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5404067 of line SALK_024253 Chr5:5404067
   
Insertion allele amplicon LBb3 - 04cn
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 04cn: 5'-TCAGATGATACAATGGGTGTTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5403619
   
wt amplicon (over InsPos) 26dk - 04cn
wt amplicon start Chr5:5403619
wt amplicon end Chr5:5404586
wt amplicon size 968 bp
Locus specific primer 04cn: 5'-TCAGATGATACAATGGGTGTTGAT-3'
2nd locus specific primer 26dk: 5'-TCTTCATGTAGAATTGGCATTCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11