Sequences from confirmation amplicon


Confirmation sequences for line SALK_024253 and hit At5g16540 are shown.
Line ID SALK_024253
Locus At5g16540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5404067
Primers used for PCR 04cn - LBb3, show primer details
   
Sequence Name 62-K108702-SALK_024253C-04cn
GenBank Accession LN485787 [GenBank]
Sequencing primer gene specific primer, 04cn: 5'-TCAGATGATACAATGGGTGTTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K108702-SALK_024253C-04cn
ATTGTGCTTATATATAAGGACTGGACTTTGTAGGTTTGGTTCAACTTGCCGATTCAATCA
TCCTCACGACCGGAAACTGGTACTGACTCTGTTTCTGTGTTTGTTCTTTCCTCTGTATCT
TGTTGACTCAAAGTTTGTAGCTTTGAGGGTCTGTGTGTGTGTGTGATTACAAGCTGATGA
ATAGTCTCGTATCCAATTTCTAGGTTATAGCAACTGCAAGGATTAAAGGAGAATACCCTG
AAAGAATTGGTCAGCCTGAATGTGAGGTAGCTCTGTTTCGAATTACTATCATAGTAATGT
CTTCTAGTTATCACATTGTCTCAAAGCTTCTATTTTTGTTGTTGTGTCTGTGCAGTTTTA
TCTGAAGACAGGGACCTGCAAATTCGGAGTGACAAAGCTGAAGACAGGATATATTGTGGT
GTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGG
GGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCC
CTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTT
GATGGTGGTTCCGAAATCGGCAAA
   
Sequence Name 54-K108701-SALK_024253C-R210
GenBank Accession LN485599 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K108701-SALK_024253C-R210
GTCATTTGTTTACCCCAATATATCCTGTCTTCAGCTTTGTCACTCCGAATTTGCAGGTCC
CTGTCTTCAGATAAAACTGCACAGACACAACAACAAAAATAGAAGCTTTGAGACAATGTG
ATAACTAGAAGACATTACTATGATAGTAATTCGAAACAGAGCTACCTCACATTCAGGCTG
ACCAATTCTTTCAGGGTATTCTCCTTTAATCCTTGCAGTTGCTATAACCTAGAAATTGGA
TACGAGACTATTCATCAGCTTGTAATCACACACACACACAGACCCTCAAAGCTACAAACT
TTGAGTCAACAAGATACAGAGGAAAGAACAAACACAGAAACAGAGTCAGTACCAGTTTCC
GGTCGTGAGGATGATTGAATCGGCAAGTTGAACCAAACCTACAAAGTCCAGTCCTTATAT
AATAAGCACAATCTGGCTCTCCGTGACGTTCCGGATAAGACCCATCAACACCCATTGTAT
CATCTGA

Last Updated on 06.12.2018 16:11