Genotyping and primer details


Genotyping and primer details for SALK_024321 - At1g76620
Line ID SALK_024321
Locus At1g76620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28757229 of line SALK_024321 Chr1:28757229
   
Insertion allele amplicon LBb3 - 02av
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 02av: 5'-CAGTGATTCAGAAAATGATCCTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28757910
   
wt amplicon (over InsPos) 10cd - 02av
wt amplicon start Chr1:28757091
wt amplicon end Chr1:28757910
wt amplicon size 820 bp
Locus specific primer 02av: 5'-CAGTGATTCAGAAAATGATCCTCA-3'
2nd locus specific primer 10cd: 5'-GCAAGTTTGCATTATGAAAGTGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11