Sequences from confirmation amplicon


Confirmation sequences for line SALK_024321 and hit At1g76620 are shown.
Line ID SALK_024321
Locus At1g76620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28757229
Primers used for PCR 02av - LBb3, show primer details
   
Sequence Name 44-K107479-SALK_024321C-02av
GenBank Accession LN485368 [GenBank]
Sequencing primer gene specific primer, 02av: 5'-CAGTGATTCAGAAAATGATCCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K107479-SALK_024321C-02av
TCTTCATATTAATTTGGAAGAGAAATGGTTGAGGCATTGGAGGAAACCGGAGAAGCAGGA
AAACAAGTGGAAGTGGTACTCGTTGATCCTCTTGAAAGTGTACAATACACAGAAGAAATG
CATCTCATGATATCTTCAGAGAGTTTATTCGGGTTTATGAAGTTGTTATGATCAACTATA
TGAGATGATCCTAAAAGCTCTCCAAGACTGTGAGTACCAGAAACTACTCTAGTCCCTTCC
TGCAAAAGAGAAAACACATAAACAAGTCACTTAAATGTTTTGTTTACAAGAAAGACTTGA
TTATAGTTAAAGTTTACCCTTGAAGATAAGGCTTTTAAACTAGTGTTGAAACTCCGAGGA
TATGAGATTGGTTTTTGGTAAGCTTGATAATGAGTTAGTGATGTTGGTGAAGTAGTCACA
GAACGAGGTAAGGTAGTAGTAACAGAGAGATTAGAGAAGGAATTAGGAAGTTGTTGCTCA
AATGATTTTCTGTAAAGAGATAAGAGATATCGTTCCATGTGTAGAATCTCTATCTCAATT
GATGCAATCTCCTTCTTCAATTCTTCTGAAGACTGAAATAAAAATCAAGATTCAAGACAT
AGTAAAACAAGAAAGCAGTAATAATATTAAACAAATTGACGCTTAGACAACTTAATAACA
CATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAC
AGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTT
TGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 60-K107480-SALK_024321C-LBb3
GenBank Accession LN485734 [GenBank]
Sequencing primer T-DNA primer, LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K107480-SALK_024321C-LBb3
GGCAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCA
GCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCCAGTACATTAAAAACGTCCGCA
ATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTAATATTATTACTGCTTTCTTGTTTT
ACTATGTCTTGAATCTTGATTTTTATTTCAGTCTTCAGAAGAATTGAAGAAGGAGATTGC
ATCAATTGAGATAGAGATTCTACACATGGAACGATATCTCTTATCTCTTTACAGAAAATC
ATTTGAGCAACAACTTCCTAATTCCTTCTCTAATCTCTCTGTTACTACTACCTTACCTCG
TTCTGTGACTACTTCACCAACATCACTAACTCATTATCAAGCTTACCAAAAACCAATCTC
ATATCCTCGGAGTTTCAACACTAGTTTAAAAGCCTTATCTTCAAGGGTAAACTTTAACTA
TAATCAAGTCTTTCTTGTAAACAAAACATTTAAGTGACTTGTTTATGTGTTTTCTCTTTT
GCAGGAAGGGACTAGAGTAGTTTCTGGTACTCACAGTCTTGGAGAGCTTTTAGGATCATC
TCATATAGTTGATCATAACAACTTCATAAACCCGAATAAACTCTCTGAAGATATCATGAG
ATGCATTTCTTCTGTGTATTGTACACTTTCAAGAGGATCAACGAGTACCACTTCCACTTG
TTTTCCTGCTTCTCCGGTTTCCTCCAATGCCTCAACCATTTTCTCTTCCAAATTTAATTA
TGAAGATAAATGGAGCTTGAATGGCGCANGTGAGGATCATT

Last Updated on 06.12.2018 16:11