Genotyping and primer details


Genotyping and primer details for SALK_024488c - At5g01650
Line ID SALK_024488c
Locus At5g01650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:243851 of line SALK_024488c Chr5:243851
   
Insertion allele amplicon LBb3 - 30um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30um: 5'-GATGGTCCTAGAGAGACAACGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:243117
   
wt amplicon (over InsPos) 34vb - 30um
wt amplicon start Chr5:243117
wt amplicon end Chr5:244113
wt amplicon size 997 bp
Locus specific primer 30um: 5'-GATGGTCCTAGAGAGACAACGAAC-3'
2nd locus specific primer 34vb: 5'-TCCTGGGTGGTCTTACTCTCTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11