Genotyping and primer details


Genotyping and primer details for SALK_024513c - At2g45695
Line ID SALK_024513c
Locus At2g45695 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18826080 of line SALK_024513c Chr2:18826080
   
Insertion allele amplicon LBb3 - 51us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 51us: 5'-TGAACATCTCAGGTCTCTCTTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18826514
   
wt amplicon (over InsPos) 33vl - 51us
wt amplicon start Chr2:18825563
wt amplicon end Chr2:18826514
wt amplicon size 952 bp
Locus specific primer 51us: 5'-TGAACATCTCAGGTCTCTCTTTGA-3'
2nd locus specific primer 33vl: 5'-CTCTCTATTTCTCCAAACCCATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11