Sequences from confirmation amplicon


Confirmation sequences for line SALK_024513c and hit At2g45695 are shown.
Line ID SALK_024513c
Locus At2g45695 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18826080
Primers used for PCR 51us - LBb3, show primer details
   
Sequence Name 03-K117668-SALK_024513C-R210
GenBank Accession LN484351 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117668-SALK_024513C-R210
CATTTGTTATAATCTTTTCNAATCGGAGTTTTGTTATTCGTAGCGTTTTTACTTTTTACC
TCACTAAAGTTCTGATTTTGACTTGGTGTCTTTTGAGATTATTTTCCTGATTGATTGTAT
GTAATACTGAAATTTAAGAACTTGAACCATGTGTGTGTTTTAGTTGTTGTTCAATGATTG
AGAAGAGTGAAACTTGACATGTGATGTGTAGGGGTGGGTTAGAGCTGCTCTGTGATTCTG
AAAAGATTCATAAAGTAAACGTTGATTTGCCCAATGGAGCTGACTCTGATGATGTAAGCT
TGAGTTCTTATGTTTTTGGATCATAAAGCTCGCAATTTTTCTCTCACTCATTCCACTTTT
CTGATTCCTGGATTAGTTTACCATGAAGCATTTGCTTTCATGGGTTCGTACAAATCTGAT
CAAAGAGAGACCTGAGATGTTC
   
Sequence Name 40-K117669-SALK_024513C-51us
GenBank Accession LN485300 [GenBank]
Sequencing primer gene specific primer, 51us: 5'-TGAACATCTCAGGTCTCTCTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117669-SALK_024513C-51us
GTAACTAATCCAGGAATCAGAAAAGTGGAATGAGTGAGAGAAAAATTGCGAGCTTTATGA
TCCAAAAACATAAGAACTCAAGCTTACATCATCAGAGTCAGCTCCATTGGGCAAATCAAC
GTTTACTTTATGAATCTTTTCAGAATCACAGAGCAGCTCTAACCCACCCCTACACATCAC
ATGTCAAGTTTCACTCTTCTCAATCATTGAACAACAACTAAAACACACACATGGTTCAAG
TTCTTAAATTTCAGTATTACATACAATCAATCAGGAAAATAATCTCAAAAGACACCAAGT
CAAAATCAGAACTTTAGTGAGGTAAAAACTAAAAACGCTACGAATAACAAAACTCCGATT
ACGAAAAGATTAATAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGA
AAATCCTGTTTGATGGTGGTTCCGA

Last Updated on 06.12.2018 16:11