Genotyping and primer details


Genotyping and primer details for SALK_024636c - At5g12010
Line ID SALK_024636c
Locus At5g12010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3878853 of line SALK_024636c Chr5:3878853
   
Insertion allele amplicon LBb3 - 63kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 63kh: 5'-AAACCTCCATTGTTAGCTCTTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3878390
   
wt amplicon (over InsPos) 03mu - 63kh
wt amplicon start Chr5:3878390
wt amplicon end Chr5:3879351
wt amplicon size 962 bp
Locus specific primer 63kh: 5'-AAACCTCCATTGTTAGCTCTTTGA-3'
2nd locus specific primer 03mu: 5'-GGATTTTTCACTTCTTTGCTTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11