Genotyping and primer details


Genotyping and primer details for SALK_024766c - At5g64850
Line ID SALK_024766c
Locus At5g64850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25922830 of line SALK_024766c Chr5:25922830
   
Insertion allele amplicon LBb3 - 23tg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23tg: 5'-GCCAATTGGTTGTAATCCATATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25923454
   
wt amplicon (over InsPos) 29uu - 23tg
wt amplicon start Chr5:25922333
wt amplicon end Chr5:25923454
wt amplicon size 1122 bp
Locus specific primer 23tg: 5'-GCCAATTGGTTGTAATCCATATCT-3'
2nd locus specific primer 29uu: 5'-TCACACATTGTTTACCCTAGTTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11