Genotyping and primer details


Genotyping and primer details for SALK_024971c - At5g13120
Line ID SALK_024971c
Locus At5g13120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4164018 of line SALK_024971c Chr5:4164018
   
Insertion allele amplicon LBb3 - 38dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38dh: 5'-AGTTCCCTACAGCCCAGGTATTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4163572
   
wt amplicon (over InsPos) 45gc - 38dh
wt amplicon start Chr5:4163572
wt amplicon end Chr5:4164347
wt amplicon size 776 bp
Locus specific primer 38dh: 5'-AGTTCCCTACAGCCCAGGTATTAT-3'
2nd locus specific primer 45gc: 5'-ATGGAATTGGTTGTTTTATTCAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11