Genotyping and primer details


Genotyping and primer details for SALK_025137c - At5g38640
Line ID SALK_025137c
Locus At5g38640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15468537 of line SALK_025137c Chr5:15468537
   
Insertion allele amplicon LBb3 - 52us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 52us: 5'-TCTGAAGGTGTAGCATCATACCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15468240
   
wt amplicon (over InsPos) 32vl - 52us
wt amplicon start Chr5:15468240
wt amplicon end Chr5:15468990
wt amplicon size 751 bp
Locus specific primer 52us: 5'-TCTGAAGGTGTAGCATCATACCTG-3'
2nd locus specific primer 32vl: 5'-AACGAGAAGATCATAGTGGCAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11