Sequences from confirmation amplicon


Confirmation sequences for line SALK_025137c and hit At5g38640 are shown.
Line ID SALK_025137c
Locus At5g38640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15468537
Primers used for PCR 52us - LBb3, show primer details
   
Sequence Name 04-K117668-SALK_025137C-R210
GenBank Accession LN484383 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117668-SALK_025137C-R210
CATTTATTAATAAAAGCGTCATGAGAGGGTCCATAATATTGCATACCAATATCTTCTCTC
GATATCAGTATCATCTTATACCGCCTTTGATTTTGTTTTACCTTACATTGGGTTTCTTGC
TTCTTTACCTTTAGGTGATCCAAATGCTATTTCAGAAGTTCATGGAAGAGAAGACATAAA
CTATTTAGATGGCTTGACCAACAACGCAAACCTGCAGTTTTTGAACTTGATGTAAGCTTG
TGTTTATCGGAGAAGTGAAACTATGTTCTGTTAATACTAATCCTCTCTGCCACTAAACTC
GTTTGTGATTTTTCTAATGACAGGTATGATGCTACACCTTCAGA
   
Sequence Name 41-K117669-SALK_025137C-52us
GenBank Accession LN485318 [GenBank]
Sequencing primer gene specific primer, 52us: 5'-TCTGAAGGTGTAGCATCATACCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K117669-SALK_025137C-52us
CAGAACATAGTTTCACTTCTCCGATAAACACAAGCTTACATCAAGTTCAAAAACTGCAGG
TTTGCGTTGTTGGTCAAGCCATCTAAATAGTTTATGTCTTCTCTTCCATGAACTTCTGAA
ATAGCATTTGGATCACCTAAAGGTAAAGAAGCAAGAAACCCAATGTAAGGTAAAACAAAA
TCAAAGGCGGTATAAGATGATACTGATATCGAGAGAAGATATTGGTATGCAATATTATGG
ACCCTCTCATGACGCTTTTATTAAATAAATTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATGGGCAAAAT

Last Updated on 06.12.2018 16:11