Genotyping and primer details


Genotyping and primer details for SALK_025156c - At3g04680
Line ID SALK_025156c
Locus At3g04680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1270261 of line SALK_025156c Chr3:1270261
   
Insertion allele amplicon LBb3 - 45fm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 45fm: 5'-CAAAGAGGTCCGTAAAAGATCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1270656
   
wt amplicon (over InsPos) 07gs - 45fm
wt amplicon start Chr3:1269825
wt amplicon end Chr3:1270656
wt amplicon size 832 bp
Locus specific primer 45fm: 5'-CAAAGAGGTCCGTAAAAGATCAAG-3'
2nd locus specific primer 07gs: 5'-TGAACCAAACAAACAGAACTAACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11