Genotyping and primer details


Genotyping and primer details for SALK_025965c - At1g76380
Line ID SALK_025965c
Locus At1g76380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28654511 of line SALK_025965c Chr1:28654511
   
Insertion allele amplicon LBb3 - 31dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 31dr: 5'-AGATTTTCTCGACCAAGACAAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28653900
   
wt amplicon (over InsPos) 15ep - 31dr
wt amplicon start Chr1:28653900
wt amplicon end Chr1:28654748
wt amplicon size 849 bp
Locus specific primer 31dr: 5'-AGATTTTCTCGACCAAGACAAAAA-3'
2nd locus specific primer 15ep: 5'-ACTCAAAAACGTTATCCCAAAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11