Genotyping and primer details


Genotyping and primer details for SALK_026061c - At1g03050
Line ID SALK_026061c
Locus At1g03050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:707710 of line SALK_026061c Chr1:707710
   
Insertion allele amplicon LBb3 - 23di
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23di: 5'-AACGCAGAGTAGTCCCAAGAATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:708144
   
wt amplicon (over InsPos) 04ep - 23di
wt amplicon start Chr1:707503
wt amplicon end Chr1:708144
wt amplicon size 642 bp
Locus specific primer 23di: 5'-AACGCAGAGTAGTCCCAAGAATTA-3'
2nd locus specific primer 04ep: 5'-ACATTGACGAAACAAACAAACAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11