Genotyping and primer details


Genotyping and primer details for SALK_026098c - At1g20540
Line ID SALK_026098c
Locus At1g20540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7113017 of line SALK_026098c Chr1:7113017
   
Insertion allele amplicon LBb3 - 30cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30cv: 5'-TATGGGACTCTAATTCCATTGACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7112415
   
wt amplicon (over InsPos) 95dy - 30cv
wt amplicon start Chr1:7112415
wt amplicon end Chr1:7113240
wt amplicon size 826 bp
Locus specific primer 30cv: 5'-TATGGGACTCTAATTCCATTGACC-3'
2nd locus specific primer 95dy: 5'-CCATATAACTCAGGAATTTGCCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11