Sequences from confirmation amplicon


Confirmation sequences for line SALK_026098c and hit At1g20540 are shown.
Line ID SALK_026098c
Locus At1g20540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7113017
Primers used for PCR 30cv - LBb3, show primer details
   
Sequence Name 03-K109680-SALK_026098C-30cv
GenBank Accession LN484331 [GenBank]
Sequencing primer gene specific primer, 30cv: 5'-TATGGGACTCTAATTCCATTGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K109680-SALK_026098C-30cv
ATAATTTAAGAAATTTGATATGTATATGTCTCTGAGTAGTTAGTGAAATTGTAACATAAG
AGATTGGGCCTGACTTTATTAAGTTAGGCCCATTAATGTCTATCACTAACACGAAAACCA
GCATCTGCTTCAGCTCGTCGTCTTCTCTACCTGAATCAGTGCGTCTGGCTCAGGTGACAA
TGCAAGGAGGATCATCGGGTATTGGATACGGTTTGAAGTATCAGGTGATTCATCATCTTC
ATATTTTCCTTAATTTTCCTTAGGAAGATTCTTTAAAGACGGAATTCATTTTGTTTCAGT
CTAGATGTATATCGGATGTGAAAGCCGATACGGATTACACAAGCTTTCTCACTGGAACTC
TTAGTTTAAAAGAAGAAAATGAGGTGAACCCATGTCTTGATTTTGATTTCGTCTTCTATT
TTAGTCGGGAAGATACTGAAAGTTTTCGTTTTTTGTTCTCCACCATTTGAGATGCTTTTA
AGGTTCATCTTCTTCGTTTGTCATCTGGTGGATCTGAGCTTATATGTGAGGGTTTGTTCT
CTCATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTT
TTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCT
TCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGC
GAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 19-K109679-SALK_026098C-R210
GenBank Accession LN484809 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K109679-SALK_026098C-R210
GCGTCATTTGTTACCCACAATATGAGAGAACAAACCCTCACATATAAGCTCAGATCCACC
AGATGACAAACGAAGAAGATGAACCTTAAAAGCATCTCAAATGGTGGAGAACAAAAAACG
AAAACTTTCAGTATCTTCCCGACTAAAATAGAAGACGAAATCAAAATCAAGACATGGGTT
CACCTCATTTTCTTCTTTTAAACTAAGAGTTCCAGTGAGAAAGCTTGTGTAATCCGTATC
GGCTTTCACATCCGATATACATCTAGACTGAAACAAAATGAATTCCGTCTTTAAAGAATC
TTCCTAAGGAAAATTAAGGAAAATATGAAGATGATGAATCACCTGATACTTCAAACCGTA
TCCAATACCCGATGATCCTCCTTGCATTGTCACCTGAGCCAGACGCACTGATTCAGGTAG
AGAAGACGACGAGCTGAAGCAGATGCTGGTTTTCGTGTTAGTGATAGACATTAATGGGCC
TAACTTAATAAAGTCAGGCCCAATCTCTTATGTTACAATTTCACTAACTACTCAGAGACA
TATACATATCAAATTTCTATAAATTATCACTACTCACTCTAGTATATATGTTACAAAACG
GGTCAATGGAATTAGAGTCCCA

Last Updated on 06.12.2018 16:11