Genotyping and primer details


Genotyping and primer details for SALK_026574c - At5g50300
Line ID SALK_026574c
Locus At5g50300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20466611 of line SALK_026574c Chr5:20466611
   
Insertion allele amplicon LBb3 - 16ey
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16ey: 5'-AACGAGTTTCGGTCTAATGAAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20467302
   
wt amplicon (over InsPos) 01go - 16ey
wt amplicon start Chr5:20466513
wt amplicon end Chr5:20467302
wt amplicon size 790 bp
Locus specific primer 16ey: 5'-AACGAGTTTCGGTCTAATGAAGAA-3'
2nd locus specific primer 01go: 5'-GACGGAAGAAACTTGATTATGCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11