Sequences from confirmation amplicon


Confirmation sequences for line SALK_026574c and hit At5g50300 are shown.
Line ID SALK_026574c
Locus At5g50300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20466611
Primers used for PCR 16ey - LBb3, show primer details
   
Sequence Name 75-K111509-SALK_026574C-16ey
GenBank Accession LN486127 [GenBank]
Sequencing primer gene specific primer, 16ey: 5'-AACGAGTTTCGGTCTAATGAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K111509-SALK_026574C-16ey
TTCGTCCGGCTATTTCTTGGATTAGAGGCACTCAAGTCACAATCTTTCCACACACTCCTC
TTGGAGACTCAAACTACAACTACTTCACAAAAATCGTTGACTTTCACAAGATCCAGTCAA
CTTTAGGAGCCATAAGCTTCACGGAGTTTAGAAAGAGCGAGGTTTGGGTCGCGTTCGCTA
CACTCTTCTACGTTGACCTTCTTGGTACGACCGGAGTACTCTACACAATGGCTGAGATCG
GAGGATTCGTGGAAGATGGGAAGTTTGAAGGAGAGTACGCGGCGTACTTAGTAGACGCCG
GCTCCTCCGTGGTGGGATCGGCGTTAGGTGTTACAACGACGGCGACTTTTGTGGAGTCTT
CGGCGGGGTTGAAAGAAGGAGGGAAAACGGGGCTAACGGCCGTTATCGTAGGGTTGTATT
TCTTGGCGTCAATGTTTTTTACGCCGTTAGTTACTAACGTGCCGAGGTGGGCGGTTGGAC
CGTCGTTGGTGATGGTTGGTGTGATGATGATGGGAGTTGTAAAAGATATTAGATGGGGAG
AGACTAAAGAAGCCGTGACAGCATTTGTAACGATNTTGCTTATGCCGTTGACTTATTCCA
TTGCTAATGGGATTATTGCTGGTATTGGTATATATCCGCATATACAAATTGACGCTTAGA
CAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAN
TGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAATTGCANCAAGCG
GTCCACGCTGGTTTGCCCCAGCAG
   
Sequence Name 61-K111508-SALK_026574C-R210
GenBank Accession LN485764 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K111508-SALK_026574C-R210
GTCATTTGTATTGCGGATATATACCAATACCAGCAATAATCCCATTAGCAATGGAATAAG
TCAACGGCATAAGCAAGATCGTTACAAATGCTGTCACGGCTTCTTTAGTCTCTCCCCATC
TAATATCTTTTACAACTCCCATCATCATCACACCAACCATCACCAACGACGGTCCAACCG
CCCACCTCGGCACGTTAGTAACTAACGGCGTAAAAAACATTGACGCCAAGAAATACAACC
CTACGATAACGGCCGTTAGCCCCGTTTTCCCTCCTTCTTTCAACCCCGCCGAAGACTCCA
CAAAAGTCGCCGTCGTTGTAACACCTAACGCCGATCCCACCACGGAGGAGCCGGCGTCTA
CTAAGTACGCCGCGTACTCTCCTTCAAACTTCCCATCTTCCACGAATCCTCCGATCTCAG
CCATTGTGTAGAGTACTCCGGTCGTACCAAGAAGGTCAACGTAGAAGAGTGTAGCGAACG
CGACCCAAACCTCGCTCTTTCTAAACTCCGTGAAGCTTATGGCTCCTAAAGTTGACTGGA
TCTTGTGAAAGTCAACGATTTTTGTGAAGTAGTTGTAGTTTGAGTCTCCAAGAGGAGTGT
GTGGAAAGATTGTGACTTGAGTGCCTCTAATCCAAGAAATAGCCGTGACGAACACGATTC
CGTAAATCATACTACCTTTGACGTTCTTCATTAGACCAAAACTCG

Last Updated on 06.12.2018 16:11