Genotyping and primer details


Genotyping and primer details for SALK_026686 - At1g69090
Line ID SALK_026686
Locus At1g69090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25978086 of line SALK_026686 Chr1:25978086
   
Insertion allele amplicon LBb3 - 53wx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 53wx: 5'-TTGGATTAGGATAGGCTCCTTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25978833
   
wt amplicon (over InsPos) 03xf - 53wx
wt amplicon start Chr1:25977496
wt amplicon end Chr1:25978833
wt amplicon size 1338 bp
Locus specific primer 53wx: 5'-TTGGATTAGGATAGGCTCCTTTAG-3'
2nd locus specific primer 03xf: 5'-AATCATTAATCCCGTTTGAAAGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11