Genotyping and primer details


Genotyping and primer details for SALK_026853 - At4g01970
Line ID SALK_026853
Locus At4g01970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:854891 of line SALK_026853 Chr4:854891
   
Insertion allele amplicon LBb3 - 18ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18ea: 5'-AGACCTTGGAGTCACTAGCAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:855357
   
wt amplicon (over InsPos) 07fg - 18ea
wt amplicon start Chr4:854427
wt amplicon end Chr4:855357
wt amplicon size 931 bp
Locus specific primer 18ea: 5'-AGACCTTGGAGTCACTAGCAGAAG-3'
2nd locus specific primer 07fg: 5'-CGAGTTCATGAAAAGGATTTCTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11