Genotyping and primer details


Genotyping and primer details for SALK_027235c - At2g43490
Line ID SALK_027235c
Locus At2g43490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18057520 of line SALK_027235c Chr2:18057520
   
Insertion allele amplicon LBb3 - 15td
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15td: 5'-GGGGTATGTTTTCACTTTGGTAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18057841
   
wt amplicon (over InsPos) 14ua - 15td
wt amplicon start Chr2:18056979
wt amplicon end Chr2:18057841
wt amplicon size 863 bp
Locus specific primer 15td: 5'-GGGGTATGTTTTCACTTTGGTAGA-3'
2nd locus specific primer 14ua: 5'-CTTTGATCTCTGGAGTGGACCTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11