Genotyping and primer details


Genotyping and primer details for SALK_027488c - At1g76350
Line ID SALK_027488c
Locus At1g76350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28641893 of line SALK_027488c Chr1:28641893
   
Insertion allele amplicon LBb3 - 37wu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 37wu: 5'-TCAGAAAATCATCCTCTTGTGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28642524
   
wt amplicon (over InsPos) 19xf - 37wu
wt amplicon start Chr1:28641334
wt amplicon end Chr1:28642524
wt amplicon size 1191 bp
Locus specific primer 37wu: 5'-TCAGAAAATCATCCTCTTGTGATG-3'
2nd locus specific primer 19xf: 5'-TGCTATGTTGGTGATCCTACAGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11