Sequences from confirmation amplicon


Confirmation sequences for line SALK_027488c and hit At1g76350 are shown.
Line ID SALK_027488c
Locus At1g76350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28641893
Primers used for PCR 37wu - LBb3, show primer details
   
Sequence Name 38-K118859-SALK_027488C-37wu
GenBank Accession LN485275 [GenBank]
Sequencing primer gene specific primer, 37wu: 5'-TCAGAAAATCATCCTCTTGTGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K118859-SALK_027488C-37wu
GATCTTGAGACTAGTCTAGTCTTATCCGTGGGATCTTCCTTGATTACTTGTGTTTCACTG
GAACAGCTTGAGCTGTGACTACACGAGGAACCAGGGGACTTTGCAGGTGGTGAATGTGAA
TAGGACAAGAATGTAGTAATCTTGGATTGCTGTTGTAGGGATGATGCTTCTGGTGAAGCA
GCTAGATTTGGGAAACTCGCGTAGAAGGAGCCTATAGGAAGATGATGACCCGAAACACCT
TCAACCGAGTCGATGACCCTTTGGATTTTCTGAAGCGAATGACCAACTTTCTTGATTTTT
CTTGAAGGCCATCTCTGTATCCCATGCTGTCTGCATATCCTCTTCAATGTCGTTGGACAA
ACTGAAACAAATTCCAAGATCAATAACAGAATCTCTGATGAATTCAAATTCCAAGAATGA
GAATGTCAATGTTTTACTTACCACCAATACTCTTGGCAGCATCTTTGAGACTCCCTGCGA
AATATTGGCGAAGAACATCCAAAGTTATGTTTTTTTCGGCCTTCGTTCGCTTTTTCTCAG
TCACTCTATTGAAACCACTAGCACTAGAGAAAGTGACAAATTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCAGTTTGATGGTGGTTCC
   
Sequence Name 14-K118858-SALK_027488C-R210
GenBank Accession LN484684 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118858-SALK_027488C-R210
GTCATTTGTCCTTTCTCAGTGCTAGTGGTTTCATAGAGTGACTGAGAAAAAGCGAACGAA
GGCCGAAAAAAACATAACTTTGGATGTTCTTCGCCAATATTTCGCAGGGAGTCTCAAAGA
TGCTGCCAAGAGTATTGGTGGTAAGTAAAACATTGACATTCTCATTCTTGGAATTTGAAT
TCATCAGAGATTCTGTTATTGATCTTGGAATTTGTTTCAGTTTGTCCAACGACATTGAAG
AGGATATGCAGACAGCATGGGATACAGAGATGGCCTTCAAGAAAAATCAAGAAAGTTGGT
CATTCGCTTCAGAAAATCCAAAGGGTCATCGACTCGGTTGAAGGTGTTTCGGGTCATCAT
CTTCCTATAGGCTCCTTCTACGCGAGTTTCCCAAATCTAGCTGCTTCACCAGAAGCATCA
TCCCTACAACAGCAATCCAAGATTACTACATTCTTGTCCTATTCACATTCACCACCTGCA
AAGTCCCCTGGTTCCTCGTGTAGTCACAGCTCAAGCTGTTCCAGTGAAACACAAGTAATC
AAGGAAGATCCCACGGATAAGACTAGACTAGTCTCAAGATCTTTTAAAGAAACGCAAACC
ACACACTTATCACCATCATCACAAGAGGATGATTTTCTGA

Last Updated on 06.12.2018 16:11