Genotyping and primer details


Genotyping and primer details for SALK_027562c - At1g17050
Line ID SALK_027562c
Locus At1g17050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5829156 of line SALK_027562c Chr1:5829156
   
Insertion allele amplicon LBb3 - 35eq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 35eq: 5'-ACTCTTTGCCTCAACCAAAACTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5829759
   
wt amplicon (over InsPos) 12ll - 35eq
wt amplicon start Chr1:5828990
wt amplicon end Chr1:5829759
wt amplicon size 770 bp
Locus specific primer 35eq: 5'-ACTCTTTGCCTCAACCAAAACTAA-3'
2nd locus specific primer 12ll: 5'-TACTTCTGATGTGGTTTTGTCTCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11