Sequences from confirmation amplicon


Confirmation sequences for line SALK_027562c and hit At1g17050 are shown.
Line ID SALK_027562c
Locus At1g17050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5829156
Primers used for PCR 35eq - LBb3, show primer details
   
Sequence Name 53-K113814-SALK_027562C-R210
GenBank Accession LN485584 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K113814-SALK_027562C-R210
CTTACACATTGGACTCTCAGTTTCCGAAGATTCTTCTCTTTGGACTCTCAGTTTCTGAAA
CTTATTATTAGCCGTCTTTTTTTGGTTTGGGTTTGCCTAAAAGTTGTCGTCACTTGTGGA
TTTTGGTCTGAGAAATGATGATGTCATGTCGGAATATAGACTTGGGTACGAGTGTTCTTG
ATCATTCTTGTAGCTCTTCTTCAACAAGTCGTCGATTCTTGTTTGGCAATTCTTCGAAGA
CCGTTTGTATGATTGGTGGCAGAAGCTGTGTTGGAAATTTGGTATTTCTCCGGCGAGATT
TGGCTACTTGCAGAGCTGTTCCGGCTAAATCCAAGGAGAATTCTCTGGTCAACGGTAACC
AATTTTTCAGCAGAACTATCTGATTTTGGTTGTGTGATTGATTGAGATTCTTTGATTTTG
AGGTTTGGTTGGTTAAGTAATTGAATCATGTTTGATTGGTCTTCACCTTTTTGGTGCATA
GTTTTAGTCTTATAACAGTTCAGTTTTGAGCTCATGAGACAGAGAGTCTCATGAATTAAA
GTTTCGAGCTTTTTGAATGTTTGCTATGGAAATAGAAATCTTTAGTTTTGGTTGAGCCAA
AGAG
   
Sequence Name 66-K113815-SALK_027562C-35eq
GenBank Accession LN485901 [GenBank]
Sequencing primer gene specific primer, 35eq: 5'-ACTCTTTGCCTCAACCAAAACTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K113815-SALK_027562C-35eq
TTCAAAGCTCGAAACTTAATTCATGAGACTCTCTGTCTCATGAGCTCAAAACTGAACTGT
TATAAGACTAAAACTATGCACCAAAAAGGTGAAGACCAATCAAACATGATTCAATTACTT
AACCAACCAAACCTCAAAATCAAAGAATCTCAATCAATCACACAACCAAAATCAGATAGT
TCTGCTGAAAAATTGGTTACCGTTGACCAGAGAATTCTCCTTGGATTTAGCCGGAACAGC
TCTGCAAGTAGCCAAATCTCGCCGGAGAAATACCAAATTTCCAACACAGCTTCTGCCACC
AATCATACAAACGGTCTTCGAAGAATTGCCAAACAAGAATCGACGACTTGTTGAAGAAGA
GCTACAAGAATGATCAAGAACACTCGTACCCAAGTCTATATTCCGACATGACATCATCAT
TTCTCAGACCAAAATCCACAAGTGACGACNACTTTTAGGCAAACCCAAACCAAAAAAAGA
CGGCTAATAATAAGTTTCAGAAACTGAGAGTCCAAAGAGAAGAATCTTCGGAAACTGAGA
GTCCAATGTGTTAAGTTTTTTTCAACAAGTTGACGCTNAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG

Last Updated on 06.12.2018 16:11