Genotyping and primer details


Genotyping and primer details for SALK_027974c - At4g25560
Line ID SALK_027974c
Locus At4g25560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13053441 of line SALK_027974c Chr4:13053441
   
Insertion allele amplicon LBb3 - 33um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33um: 5'-ACTTCACGGCCATTATCACTATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13054014
   
wt amplicon (over InsPos) 31vl - 33um
wt amplicon start Chr4:13052875
wt amplicon end Chr4:13054014
wt amplicon size 1140 bp
Locus specific primer 33um: 5'-ACTTCACGGCCATTATCACTATTC-3'
2nd locus specific primer 31vl: 5'-GTTTCATTCTTCCTTGGGTAACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11