Genotyping and primer details


Genotyping and primer details for SALK_028065 - At1g43560
Line ID SALK_028065
Locus At1g43560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:16398720 of line SALK_028065 Chr1:16398720
   
Insertion allele amplicon LBb3 - 19ht
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19ht: 5'-GTTGAGGCTTTATTGTTGCTTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:16397943
   
wt amplicon (over InsPos) 03jm - 19ht
wt amplicon start Chr1:16397943
wt amplicon end Chr1:16398927
wt amplicon size 985 bp
Locus specific primer 19ht: 5'-GTTGAGGCTTTATTGTTGCTTTTC-3'
2nd locus specific primer 03jm: 5'-TCAACTCTTTTGATGATTTGTTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11