Sequences from confirmation amplicon


Confirmation sequences for line SALK_028065 and hit At1g43560 are shown.
Line ID SALK_028065
Locus At1g43560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16398720
Primers used for PCR 19ht - LBb3, show primer details
   
Sequence Name 26-K112874-SALK_028065C-19ht
GenBank Accession LN485034 [GenBank]
Sequencing primer gene specific primer, 19ht: 5'-GTTGAGGCTTTATTGTTGCTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K112874-SALK_028065C-19ht
AGAGATTTATGCTAATCTCTTGTAATCGTCTTTGTGTGAGAGCTAAGAACGATCATATCT
TGTGATTTCTGCAGTTACAACCTTTGTAATTTGTAGATCTCATATTATTGATTATCATAG
AGTTTGGGTTTTACAATCGGAGTAGCTTAAACTTATCATTTGGTCGGATACGTTTGTATG
TATTGAGCATCCCTAAGTAGGAAAAACTTTTAAAAATGAACAAGAGTTTTAAGAAAAATG
GCAGATGCCATATATGAATAGGTAGATGATTATACCGAGCAAACAATCAACAATGGCTTA
TGTCACAACAGATTGTTCATAGATGACAAGTTTTCTTGGTTTCTCAAACCTACTGTTTCA
CTTGCAAAGAATTCTCAATACGCTCAACGAGTTGGTTCGCTGGCAAGGCACCCTCCTGCA
AGAACCCAAAAGCAAAACTTGTCAACAAAAGTTAAAAACTTGTGCTATAAAGAAAACAGC
TCTGTCTTATAAGCTTTTCAAGATAAGCTTTTAAGTGTAGCTACAATTGAGCCTTTGATG
CAAGGAGTAGATATGCCGTATTCATGCGTGATAGAAAACTTACGAAACGGTCCCAGAGTT
TGCCGTCTTTGAATAAGATGAATGTGGGCAAAGCCTCAATCTGGTATTTGTTAGCAAGAC
TTGGGTACTTCTCAGTATCAATTTTCACAACTGCAATTATATCTNTCAGTGCTCAGGATA
TATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGTTTGATGGTGGTTCC
   
Sequence Name 18-K112873-SALK_028065C-R210
GenBank Accession LN484781 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K112873-SALK_028065C-R210
CAATATATCCTGAGCACTGAAAGATATAATTGCAGTTGTGAACCTTTATACTGAGAAGTA
CCCAAGTCTTGCTAACAAATACCAGATTGAGGCTTTGCCCACATTCATCTTATTCAAAGA
CGGCAAACTCTGGGACCGTTTCGTAAGTTTTCTATCACGCATGAATACGGCATATCTACT
CCTTGCATCAAAGGCTCAATTGTAGCTACACTTAAAAGCTTATCTTGAAAAGCTTATAAG
ACAGAGCTGTTTTCTTTATAGCACAAGTTTTTAACTTTTGTTGACAAGTTTTGCTTTTGG
GTTCTTGCAGGAGGGTGCCTTGCCAGCGAACCAACTCGTTGAGCGTATTGAGAATTCTTT
GCAAGTGAAACAGTAGGTTTGAGAAACCAAGAAAACTTGTCATCTATGAACAATCTGTTG
TGACATAAGCCATTGTTGATTGTTTGCTCGGTATAATCATCTACCTATTCATATATGGCA
TCTGCCATTTTTCTTAAAACTCTTGTTCATTTTTAAAAGTTTTTCCTACTTAGGGATGCT
CAATACATACAAACGTATCCGACCAAATGATAAGTTTAAGCTACTCCGATTGTAAAACCC
AAACTCTATGATAATCAATAATATGAGATCTACAAATTACAAAGGTTGTAACTGCAGAAA
TCACAAGATATGATCGTTCTTAGCTCTCACACAAAGACGATTACAAGAGATTAGCATAAA
TCTCTCTACTGGCACATCAGTAGGCTAAAAGTCTCAGCATAAAAGTATGAAAAGCAACAA
TAAGGCCTCA

Last Updated on 06.12.2018 16:11